Table 1.

Differences in iPSC Reprogramming Methods

FeatureRNA TransfectionSendai VirusEpisomal VectorsLentivirus
Efficiency (with fibroblasts)HighModerateLowHigh
Reliability (with fibroblasts)ModerateHighHighHigh
Amount of workHighLowLowVery high (requires excision of vector)
Aneuploidy rateLowModerateHighModerate
Number of input cells (fibroblasts)LowHighHighModerate
Time to iPSC colony emergenceLowModerateModerateModerate
Efficiency (blood cells)Not reportedHighHighHigh
Reliability (blood cells)Not reportedHighHighHigh
Special equipment needs5% O2 incubatorNoneTransfection device and O2 incubatorNone
Proportion of iPSC lines free of reprogramming reagents by passage 5Very highNoneModerateNone
Proportion of iPSC lines free of reprogramming reagents by passage 9–11Very highHighModerateNone
  • iPSC indicates induced pluripotent stem cell.

  • Adapted from Schlaeger et al29 by permission from Macmillan Publishers Ltd: Nature Biotechnology. Copyright © 2014, Springer Nature.